Light-sheet fluorescence microscopy of ECi cleared organs. Optical clearing via ECi allows LSFM of soft and hard murine organs, as bones (calvaria, left), kidney (middle) and heart (right). While autofluorescent signals provide detailed information of general tissue composition, specific antibody-labeling enables the visualization of defined cell types, as e.g. endothelial staining (CD31). (By Anika Klingberg, Publication)

Microglia in the brain of a CX3CR1-GFP mouse. In-situ 2-Photon imaging of a CX3CR1-GFP mouse brain shows GFP+ microglia in the tissue below the meninges (SHG) and the brain vasculature (CD31). (By Anika Klingberg, Publication)

Visualisation of NETs by bicoloured dSTORM. Human Neutrophils were stimulated with PMA for 3 hours at 37°C. Afterwards, the cells were fixed with 4% PFA. Histones H1 and Neutrophil Elastase were stained with antibodies. Consequently the MEA-buffer was added, which brought the fluochromes in a blinking state. (By Alexandra Brenzel and Jacqueline Heinen-Weiler)

PMA-induced NETosis of human neutrophils. 2-Photon imaging of NETosis events induced via PMA-stimulation. The extracellular traps are stained with Sytox-Orange, while the activation of neutrophils is indicated via the expression of CD66b on the cell surface. (By Anika Klingberg and Manuel Stecher)

Alveolar macrophages in the lung. In-situ 2-Photon imaging of a MacBlue-eCFP mouse lung shows CFP+ alveolar macrophages in the alveoli of the lung (SHG). (By Sophie Henneberg)

Light-sheet fluorescence microscopy of ECi cleared kidneys. Optical sections of an ECi cleared and LSFM-visualized entire murine kidney show endothelial structures (CD31), as the interlobar vessels and glomerular capillary tufts. (By Anika Klingberg, Publication)

Visualisation of NETs with central zoom in via SEM (= Scanning Electron Microscopy). Human Neutrophils were stimulated with PMA and E.coli added. They were incubated for 3 hours at 37°C to induce NETs, which caught the E.coli. (Inlens 50X, SE2 1.01KX, SE2 29.27KX) (By Jacqueline Heinen-Weiler)

In vitro hyphal cell wall staining of the fungus Aspergillus fumigatus. Aspergillus fumigatus tdTomato is stained with the fluorophore coupled antibody JF5 Dylight650 and the nucleus with DAPI. The fungus was grown for 16h after conidia seeding. (By Djamschid Solouk)

Combined confocal and 2-photon laser scanning microscopy of glomerular capillary tufts. Sequential confocal and 2-Photon laser scanning microscopy allows the visualization of endothelial structures (CD31) in healthy (left) and NTN-affected (right) glomerular capillary tufts in combination with SHG signal of the Bowman’s capsule in the autofluorescent kidney tissue (Scalebars = 20 µm). (By Anika Klingberg, Publication)

Visualisation of NETs via SEM (= Scanning Electron Microscopy). Human Neutrophils were stimulated with PMA and E.coli added. They were incubated for 3 hours at 37°C to induce NETs, which caught the E.coli. Afterwards the image was colored using Photoshop (Adobe). (By Jacqueline Heinen-Weiler)

MIP and 3D reconstructions of murine testis imaged using light sheet fluorescence microscopy. Autofluorescence and Endothelia are depicted. (By Simon Merz, Sophie Henneberg and Sebastian Korste)